Effect of Raspberry bushy dwarf virus, Raspberry leaf mottle virus, and Raspberry latent virus on Plant Growth and Fruit Crumbliness in ‘Meeker’ Red Raspberry
نویسندگان
چکیده
منابع مشابه
Real-time RT-PCR for detection of Raspberry bushy dwarf virus, Raspberry leaf mottle virus and characterizing synergistic interactions in mixed infections.
Two TaqMan-based real-time One-Step RT-PCR assays were developed for the rapid and efficient detection of Raspberry bushy dwarf virus (RBDV) and Raspberry leaf mottle virus (RLMV), two of the most common raspberry viruses in North America and Europe. The primers and probes were designed from conserved fragments of the polymerase region of each virus and were effective for the detection of diffe...
متن کاملComplete Nucleotide Sequence and Properties of Raspberry Mottle Virus
During routine graft indexing of Rubus clones at the USDA-ARS Horticultural Crops Research Laboratory in Corvallis, Oregon, a ‘Glen Clova’ plant that originated in Scotland induced severe symptoms including stunting and apical necrosis when grafted on ‘Munger’ black raspberry (Rubus occidentalis). Cloning of viral dsRNA obtained from the plant and sequence analysis indicated the presence of a n...
متن کاملSingle-step immunocapture RT-PCR in the detection of raspberry bushy dwarf virus.
An immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) method for a highly sensitive analysis of raspberry bushy dwarf virus (RBDV) in infected plants is described. In the method, preliminary purification of virus particles or viral RNA from the plant material is not necessary. Viruses are enriched during the assay by antibodies bound in the PCR microplate wells, followed ...
متن کاملThe nucleotide sequence of RNA-1 of raspberry bushy dwarf virus.
Raspberry bushy dwarf virus (RBDV) has isometric, 33 nm diameter particles and a bipartite RNA genome. Sequencing of the larger component (RNA-1) showed that it consists of 5449 nucleotides and contains one large open reading frame encoding a putative translation product with a calculated M(r) of 190,000. Comparisons of this polypeptide with non-structural proteins of other plant viruses reveal...
متن کاملIdentification, Characterization, and Detection of Black raspberry necrosis virus.
ABSTRACT A serious disease was observed in black raspberry (Rubus occidentalis) in Oregon in the last decade. Plants showing mosaic symptoms declined rapidly and, in many cases, died after several years. Double-stranded RNA extraction from symptomatic black raspberry revealed the presence of two high molecular weight bands which were cloned and sequenced. Sequence analysis disclosed the presenc...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2014
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-05-13-0562-re